Insertion junction: LMJ.RY0402.163938_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.163938
Chromosome:chromosome 17
Location:5653817
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g738400 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTGGACCGCTCCCCACATCCCCCAGTCC
Internal bar code:TAGGCAGGCAGGAGGCGGATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:171
LEAP-Seq percent confirming:97.5207
LEAP-Seq n confirming:472
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTCTTGACCACGGTTG
Suggested primer 2:TACCGGAAACAACAACGACA