| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.163960 |
| Chromosome: | chromosome 9 |
| Location: | 2876308 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388450 | ELG29 | Exostosin-like glycosyltransferase 29; (1 of 2) PTHR31389:SF4 - PROTEIN F32B4.1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTACCTGGCGGCCCGGCGAGGTGGACTGC |
| Internal bar code: | ACTGGGCTGACTCGCTCTGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 210 |
| LEAP-Seq percent confirming: | 99.6226 |
| LEAP-Seq n confirming: | 1584 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAGTACAGCAGGCGCGTT |
| Suggested primer 2: | AGGTGGAGAAGTTGTGGTGC |