Insertion junction: LMJ.RY0402.163973_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.163973
Chromosome:chromosome 10
Location:1050613
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g425251 (1 of 5) IPR008274 - Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGGAAGCTGTGGCTCCGCTCCTGCAGGC
Internal bar code:GCGCAGTATATATGTTTGCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:877
LEAP-Seq percent confirming:98.3271
LEAP-Seq n confirming:2645
LEAP-Seq n nonconfirming:45
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGACCATGTACCGAAGAC
Suggested primer 2:GTCTGCTCCTCACTCGACCT