| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.163996 |
| Chromosome: | chromosome_9 |
| Location: | 2812523 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g388850 | ACA1 | P-type ATPase/cation transporter, plasma membrane | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAAGATAGGAGCCCTGGACGGAGGCAAAAA |
| Internal bar code: | CATCGCATTCTCTATCGTTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 99.7523 |
| LEAP-Seq n confirming: | 3222 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTGACACGACACTTCCC |
| Suggested primer 2: | TGAGGGTATTTTGAGTGGGC |