| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.164006 |
| Chromosome: | chromosome 12 |
| Location: | 2841149 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g502350 | HEL50 | (1 of 1) K10899 - ATP-dependent DNA helicase Q1 [EC:3.6.4.12] (RECQL); DEAD/DEAH box DNA/RNA helicase, related to RECQ1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCCCCCCTGCTGCCCCGCACCTCATAG |
| Internal bar code: | GGGCGGAGTCGGGGTTGGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 845 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 382 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGTCTGCATGGTAGTGG |
| Suggested primer 2: | GCAGGTTCTGGCTCTCAATC |