Insertion junction: LMJ.RY0402.164008_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.164008
Chromosome:chromosome 2
Location:2846645
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g094700 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTGGAACGACTGCGGGTCGCGGCAGTCT
Internal bar code:ACATCACGGGTGACCAAATCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1185
LEAP-Seq percent confirming:99.7753
LEAP-Seq n confirming:888
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTGCTGTAAGTTTGTGGA
Suggested primer 2:GTAGGTGTGCGACCAGGAGT