| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.164035 |
| Chromosome: | chromosome 3 |
| Location: | 5347228 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g184200 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGCCCGCCAGTGCCGCATCCAGCCCGC |
| Internal bar code: | TTGTCTGGACAGCCGGTCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 938 |
| LEAP-Seq percent confirming: | 56.4433 |
| LEAP-Seq n confirming: | 219 |
| LEAP-Seq n nonconfirming: | 169 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGACTTGACCTGGATGCTG |
| Suggested primer 2: | GATGAGGGCACTGTGTCTGA |