| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.164038 |
| Chromosome: | chromosome 1 |
| Location: | 5476768 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038550 | SQD2 | Sulfoquinovosyldiacylglycerol synthase; (1 of 2) K06119 - sulfoquinovosyltransferase (SQD2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTTACCATGCCTGGATCGACATATTGC |
| Internal bar code: | GGGTTGCAGCATGTTTGCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 47 |
| LEAP-Seq percent confirming: | 99.4143 |
| LEAP-Seq n confirming: | 679 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCGTACAGGAAGCCAGTC |
| Suggested primer 2: | CAGGTGTGGAAGAAGGGTGT |