| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.164040 |
| Chromosome: | chromosome 5 |
| Location: | 965981 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246900 | (1 of 1) IPR008972//IPR028096 - Cupredoxin // EfeO-type cupredoxin-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTAGGGCTGTGCTAGGAGGCAACCACC |
| Internal bar code: | ACAGTTACCAGAGCCAGAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1071 |
| LEAP-Seq percent confirming: | 99.254 |
| LEAP-Seq n confirming: | 5588 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCATGATCGTCAACAAGG |
| Suggested primer 2: | GCAAGCCGAAGTTCCTAATG |