| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.164040 |
| Chromosome: | chromosome 5 |
| Location: | 965991 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246900 | (1 of 1) IPR008972//IPR028096 - Cupredoxin // EfeO-type cupredoxin-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGAGAGCGGCCACCGTGCACTCCTACA |
| Internal bar code: | GGACCGAGGCCGGCATCCGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 490 |
| LEAP-Seq percent confirming: | 99.6416 |
| LEAP-Seq n confirming: | 5560 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCATGATCGTCAACAAGG |
| Suggested primer 2: | GCAAGCCGAAGTTCCTAATG |