| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.164051 |
| Chromosome: | chromosome_3 |
| Location: | 5632806 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g187200 | LC6,DLL2,ODA13 | Flagellar outer arm dynein light chain 6, LC6 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAATGGCGCTCATCCAAACGTCAAGTCATG |
| Internal bar code: | CCCTGAAGACTTTGGTGCATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 362 |
| LEAP-Seq percent confirming: | 95.4627 |
| LEAP-Seq n confirming: | 2672 |
| LEAP-Seq n nonconfirming: | 127 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGATGACTGCTACGAGGTG |
| Suggested primer 2: | CGTGAGCGTATCACGAAGAA |