Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.164051 |
Chromosome: | chromosome_13 |
Location: | 3741363 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g589450 | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTCCAACTTCCGGCGCAGCTGCCCGCGTGC |
Internal bar code: | ACGTGTATGTTCTGTGGGGAGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 425 |
LEAP-Seq percent confirming: | 99.5673 |
LEAP-Seq n confirming: | 4372 |
LEAP-Seq n nonconfirming: | 19 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGGTAGCTGAACTCCACCG |
Suggested primer 2: | AACATGCCTGGTTGTAAGCC |