| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.164059 |
| Chromosome: | chromosome 5 |
| Location: | 2642482 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236450 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGAGGGGTTACGCGGGAAGGGAGGGCAC |
| Internal bar code: | GTTAGGGGATGGTGGTGTGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 286 |
| LEAP-Seq percent confirming: | 99.7721 |
| LEAP-Seq n confirming: | 5691 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGTGTGCCGAAGTAGAT |
| Suggested primer 2: | AATCATCGTCTTGACGACCC |