| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.164065 |
| Chromosome: | chromosome 8 |
| Location: | 1458789 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364550 | (1 of 3) K10587 - ubiquitin-protein ligase E3 A (UBE3A, E6AP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGCACTCAGCAGCGGCCTGCACAGAC |
| Internal bar code: | GATGCATGTGGTGGGCTTGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 272 |
| LEAP-Seq percent confirming: | 38.9359 |
| LEAP-Seq n confirming: | 322 |
| LEAP-Seq n nonconfirming: | 505 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTGTGTGTGCGTGT |
| Suggested primer 2: | AACTAACAGCAGCAGCAGCA |