| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.164071 |
| Chromosome: | chromosome 11 |
| Location: | 743830 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467630 | AMI2 | putative amidase; (1 of 2) 3.5.1.4 - Amidase / Acylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTTCACCTGTGTGTGTGTGTGTGTGTA |
| Internal bar code: | GTACCTAGTGCCACAATTTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 386 |
| LEAP-Seq percent confirming: | 99.6278 |
| LEAP-Seq n confirming: | 23287 |
| LEAP-Seq n nonconfirming: | 87 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTCCTTTGCTGACATGC |
| Suggested primer 2: | GTGTGTGTTCATGTAGGGCG |