Insertion junction: LMJ.RY0402.164095_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.164095
Chromosome:chromosome 3
Location:1926281
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g155300 PHC29 (1 of 1) IPR000095//IPR024616 - CRIB domain // Pherophorin; Putative pherophorin-chlamydomonas homolog 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCTTAACCAACACCACGCACCAACCTA
Internal bar code:GCATTGCGAGGGGGACGCGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:971
LEAP-Seq percent confirming:99.8167
LEAP-Seq n confirming:2178
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TGATGACGACAGCTCGACTC
Suggested primer 2:CTCCTCCAAGTACAGCAGCC