Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.164111 |
Chromosome: | chromosome 12 |
Location: | 9608058 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g550277 | MRS2 | Mg2+ transporter protein, CorA-like and putative mitochondrial splicing factor | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAAAGCTCACAACAATAGAGAAAGGCGT |
Internal bar code: | CGAGGTCTGTGTTCAGTGAAGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 203 |
LEAP-Seq percent confirming: | 99.7618 |
LEAP-Seq n confirming: | 10051 |
LEAP-Seq n nonconfirming: | 24 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AATGAACCTCACCAACAGGC |
Suggested primer 2: | TACACTCTGGTGCACCTTGC |