Insertion junction: LMJ.RY0402.164111_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.164111
Chromosome:chromosome 12
Location:9608058
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g550277 MRS2 Mg2+ transporter protein, CorA-like and putative mitochondrial splicing factor 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAAAAGCTCACAACAATAGAGAAAGGCGT
Internal bar code:CGAGGTCTGTGTTCAGTGAAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:203
LEAP-Seq percent confirming:99.7618
LEAP-Seq n confirming:10051
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AATGAACCTCACCAACAGGC
Suggested primer 2:TACACTCTGGTGCACCTTGC