| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.164131 |
| Chromosome: | chromosome 3 |
| Location: | 8839518 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206705 | (1 of 3) PF10250 - GDP-fucose protein O-fucosyltransferase (O-FucT) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGAGCAGGCGGCCCGCAGCTGCAAGAAC |
| Internal bar code: | ATTCGGGAGTGACTGACGCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 97.8052 |
| LEAP-Seq n confirming: | 1426 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGTTTCCCTTTCCACAC |
| Suggested primer 2: | CCAGATCTGCCTCCGAGTAG |