Insertion junction: LMJ.RY0402.164133_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.164133
Chromosome:chromosome 6
Location:7044708
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGCGCGTGCCGGCAATGCATTTTGGCACG
Internal bar code:GTTGCGGGACAGCCCTCGCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1003
LEAP-Seq percent confirming:98.4076
LEAP-Seq n confirming:309
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGTAGGAAACATGGTTGCGG
Suggested primer 2:AATCTGAACCTGAACCCACG