| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.164134 |
| Chromosome: | chromosome 2 |
| Location: | 3583076 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095135 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGTCCATGGAACACCCACGCGACCCTG |
| Internal bar code: | ACGGTTTCTGCGTAGTGAAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 955 |
| LEAP-Seq percent confirming: | 94.4249 |
| LEAP-Seq n confirming: | 43748 |
| LEAP-Seq n nonconfirming: | 2583 |
| LEAP-Seq n unique pos: | 152 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGGTGTCACTCAGTCCT |
| Suggested primer 2: | GGGTGAGTCAGTGAGTGGGT |