Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.164136 |
Chromosome: | chromosome_10 |
Location: | 1652050 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g429750 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGTGGGAAAGGGGCAGGCGGGTGGGTACTG |
Internal bar code: | TTAGACTTGACTCCGGCGTTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 264 |
LEAP-Seq percent confirming: | 99.6292 |
LEAP-Seq n confirming: | 13973 |
LEAP-Seq n nonconfirming: | 52 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGTGTGTGTGTGGTGTGTGT |
Suggested primer 2: | GTGCTACCACCTGTCTGCAA |