Insertion junction: LMJ.RY0402.164136_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.164136
Chromosome:chromosome_10
Location:1652050
Confidence (%):95
Locus disrupted Locus common name Defline Orientation Feature
Cre10.g429750 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):CGTGGGAAAGGGGCAGGCGGGTGGGTACTG
Internal bar code:TTAGACTTGACTCCGGCGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:264
LEAP-Seq percent confirming:99.6292
LEAP-Seq n confirming:13973
LEAP-Seq n nonconfirming:52
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTGTGTGTGGTGTGTGT
Suggested primer 2:GTGCTACCACCTGTCTGCAA