Insertion junction: LMJ.RY0402.164142_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.164142
Chromosome:chromosome 2
Location:6845782
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g119450 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGCTGTGGGCTGACGCGCGGGGTCACGG
Internal bar code:TACACTTTATGCTGTCAATTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1182
LEAP-Seq percent confirming:99.13
LEAP-Seq n confirming:8888
LEAP-Seq n nonconfirming:78
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTTTGTGAGCAAGGGGTT
Suggested primer 2:TTGAGTGAATGGTTGGGTGA