Insertion junction: LMJ.RY0402.164177_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.164177
Chromosome:scaffold 19
Location:33545
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre19.g750347 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCCTCTGGATAGCGGCAGCTTACTTGTC
Internal bar code:GTTCACGTAAGAGGTCGCCGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:635
LEAP-Seq percent confirming:99.9034
LEAP-Seq n confirming:3104
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGGTGGTCAGATAGGGAG
Suggested primer 2:AGGATGAATGACAGGTTGGC