| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.164184 |
| Chromosome: | chromosome 1 |
| Location: | 514937 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g002861 | SRR24 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTAAGCAAACGGCACGTGGGACGGCTG |
| Internal bar code: | GCGGCGTCGGGCAAGCAGAAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 785 |
| LEAP-Seq percent confirming: | 98.5104 |
| LEAP-Seq n confirming: | 2513 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCTGCCTGCATACTGCT |
| Suggested primer 2: | ATACGCTTGCCATAACCAGG |