| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.164185 |
| Chromosome: | chromosome 15 |
| Location: | 1057021 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g638551 | OPR78 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTACACTACAACTGGGCTGCGTTTCACTA |
| Internal bar code: | AGAGTACGCCCTGATATCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1093 |
| LEAP-Seq percent confirming: | 98.9846 |
| LEAP-Seq n confirming: | 6921 |
| LEAP-Seq n nonconfirming: | 71 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACCAGAAAACCACCAAC |
| Suggested primer 2: | CTTGGTCCGTGCGTAGGTAT |