| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.164220 |
| Chromosome: | chromosome 10 |
| Location: | 6056627 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g463300 | (1 of 1) PTHR31515:SF0 - TRANSMEMBRANE PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGGGAGCGGTGGGGTGGAGGCAGGAGAA |
| Internal bar code: | CAGCGATTTCGTGTTAATATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 898 |
| LEAP-Seq percent confirming: | 99.7102 |
| LEAP-Seq n confirming: | 4473 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAAACCACAAACACGCACA |
| Suggested primer 2: | TACAAGGTTGATAAGGCGGC |