| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.164270 |
| Chromosome: | chromosome 3 |
| Location: | 6446443 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194650 | AOA1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTACTGTCACGTGGTGCCGTCGTGGTGT |
| Internal bar code: | CATTATACGACTGGTCGCGCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1003 |
| LEAP-Seq percent confirming: | 98.2471 |
| LEAP-Seq n confirming: | 4540 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTAGGTTGGACAGCAGAT |
| Suggested primer 2: | CAACATTACTCATCCCGGCT |