| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.164280 |
| Chromosome: | chromosome 9 |
| Location: | 4835323 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397549 | (1 of 1) PF06972 - Protein of unknown function (DUF1296) (DUF1296) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCAGGCTCCAGGGTGGAAGAAATTTGC |
| Internal bar code: | TCGTGAAGGTAATCGAGCGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 99.8428 |
| LEAP-Seq n confirming: | 635 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGGAATCAGTAGCAGCA |
| Suggested primer 2: | TCACCACAGGTGGGTCTGTA |