| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.164291 |
| Chromosome: | chromosome 17 |
| Location: | 2019382 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g711401 | (1 of 10) IPR006593 - Cytochrome b561/ferric reductase transmembrane | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCATTCCACAAACGCGTATTGTTTCCCG |
| Internal bar code: | GAGGTGCCGGTGAACCGTCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 422 |
| LEAP-Seq percent confirming: | 97.4138 |
| LEAP-Seq n confirming: | 113 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTGTGCCAAGACTGTAA |
| Suggested primer 2: | TTTGGTTTTTAGCCAGGTCG |