| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.164321 |
| Chromosome: | chromosome 11 |
| Location: | 2502122 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475750 | (1 of 1) PF04187 - Haem-binding uptake, Tiki superfamily, ChaN (Cofac_haem_bdg) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCGCATGAGATGTCACCGGAACGCCAA |
| Internal bar code: | AGTGTACGTCTTTTCTCACCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 378 |
| LEAP-Seq percent confirming: | 99.0205 |
| LEAP-Seq n confirming: | 6369 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTCGTTCTGACAGTAGCA |
| Suggested primer 2: | AAGATGTGGAGGAGGTGGTG |