| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.164322 |
| Chromosome: | chromosome 3 |
| Location: | 4051286 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172451 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCCATGATTCCCAGCCAGGCCAAACTT |
| Internal bar code: | CGAGGCTCCGCATTTACTGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 550 |
| LEAP-Seq percent confirming: | 91.3083 |
| LEAP-Seq n confirming: | 3015 |
| LEAP-Seq n nonconfirming: | 287 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCCTTGGCCATCGTCTTA |
| Suggested primer 2: | GAGTCAGCCAAGACCAGGAG |