Insertion junction: LMJ.RY0402.164329_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.164329
Chromosome:chromosome 15
Location:141080
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre15.g635000 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGTTAACTCCAACCTGTACGACACGTCG
Internal bar code:CGGCCCGTGTCGAGTCCAGATT

Confirmation - LEAP-Seq

LEAP-Seq distance:303
LEAP-Seq percent confirming:99.823
LEAP-Seq n confirming:1128
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTCTCATCCACATCAGCA
Suggested primer 2:GTGGGGGTAGGAAGTGGTG