Insertion junction: LMJ.RY0402.164336_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.164336
Chromosome:chromosome 8
Location:4184708
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g380151 (1 of 1) PF00628//PF15613//PF15614 - PHD-finger (PHD) // WSTF, HB1, Itc1p, MBD9 motif 2 (WHIM2) // WSTF, HB1, Itc1p, MBD9 motif 3 (WHIM3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTGACGTCTCCTCTCTCTCCAAGCTTCC
Internal bar code:GCCAGCGCCCGCATCGGGGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:178
LEAP-Seq percent confirming:95.4595
LEAP-Seq n confirming:883
LEAP-Seq n nonconfirming:42
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGGACAAGGAGGTGAGAG
Suggested primer 2:GCCTACCCTTGTGTCACGAT