| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.164338 |
| Chromosome: | chromosome 2 |
| Location: | 6591469 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g117200 | Putative beta-galactosidase; (1 of 5) 3.2.1.23 - Beta-galactosidase / Lactase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAAAGCGCCGATGCGAAATCCCCCGTGC |
| Internal bar code: | CCGGCTGGTTCCGAGTCCGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 235 |
| LEAP-Seq percent confirming: | 98.7842 |
| LEAP-Seq n confirming: | 325 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTCACTGCACACTCTTG |
| Suggested primer 2: | CGGTGTGTGTGTGTGTGTGT |