Insertion junction: LMJ.RY0402.164339_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.164339
Chromosome:chromosome 16
Location:4491182
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g667451 TLP3,CAV6 Tubby-like protein; (1 of 5) PTHR10037//PTHR10037:SF62 - VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM // SODIUM CHANNEL PROTEIN PARA CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATGCTGCCGCCAATCATGCTGCCGCCCA
Internal bar code:CCGACAGTGCTAAACGATGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:141
LEAP-Seq percent confirming:91.757
LEAP-Seq n confirming:423
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TCATGCCGTCGTACTCCATA
Suggested primer 2:AGCCAGCCTAGGTGTGTACC