| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.164347 |
| Chromosome: | chromosome_16 |
| Location: | 4656158 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g687450 | CPLD54 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGCCACTGGACCAGTGAGGCAACCGTGTGT |
| Internal bar code: | ATGACGACGTGGAGATCTCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 99.277 |
| LEAP-Seq n confirming: | 5355 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATAAGCTTCGTCACTGCCA |
| Suggested primer 2: | CAGGCCAGAGAGAGGGAAG |