| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.164358 |
| Chromosome: | chromosome 16 |
| Location: | 7353830 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684715 | DCL2 | Dicer-like protein; (1 of 4) 3.1.26.3 - Ribonuclease III / RNase III | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTACAATGCACAACTTCAACGACTTCGG |
| Internal bar code: | GCTGATCTCCTGTTGTACATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 347 |
| LEAP-Seq percent confirming: | 98.3183 |
| LEAP-Seq n confirming: | 7191 |
| LEAP-Seq n nonconfirming: | 123 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATCAGACCAACAGCAGCA |
| Suggested primer 2: | TGCATTCGGAGTGCTCTATG |