| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.164387 |
| Chromosome: | chromosome 12 |
| Location: | 3084384 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCAGCAACCGGGAGCTCCAGGCATGTGT |
| Internal bar code: | CGGGGGTGAATTGTCGCGCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 139 |
| LEAP-Seq percent confirming: | 97.54 |
| LEAP-Seq n confirming: | 1705 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATGTACCTCCACGGCTC |
| Suggested primer 2: | AGCACATGGAGGAGGTGAAC |