| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.164387 |
| Chromosome: | chromosome 12 |
| Location: | 4430533 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521200 | RFC1 | (1 of 1) K10754 - replication factor C subunit 1 (RFC1); DNA replication factor C complex subunit 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGGTCCTGGCCTCGAGACTTAGTTGCC |
| Internal bar code: | TTTACAACCCTGTTAAACGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 376 |
| LEAP-Seq percent confirming: | 99.1411 |
| LEAP-Seq n confirming: | 24816 |
| LEAP-Seq n nonconfirming: | 215 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTCTGATGTGGCTGTGT |
| Suggested primer 2: | CCATTATTCAGGAGTGCGGT |