Insertion junction: LMJ.RY0402.164412_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.164412
Chromosome:chromosome 17
Location:4799182
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g733800 (1 of 139) IPR011011 - Zinc finger, FYVE/PHD-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGATCCAGATTGTGGGGCGCCTGGGCGGC
Internal bar code:TCTTCGGACTCGCGCCTGGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:300
LEAP-Seq percent confirming:98.4894
LEAP-Seq n confirming:326
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCTCCTCCTCCTCGTCAT
Suggested primer 2:CAGCTGTCATCATCGCAATC