Insertion junction: LMJ.RY0402.164412_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.164412
Chromosome:chromosome 17
Location:4799186
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g733800 (1 of 139) IPR011011 - Zinc finger, FYVE/PHD-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCGAACCAGGATTCAGTTGCTCGAGCTGA
Internal bar code:GACGATTGCGGTTGGGGCCCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:234
LEAP-Seq percent confirming:99.2701
LEAP-Seq n confirming:272
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCTCCTCCTCCTCGTCAT
Suggested primer 2:CAGCTGTCATCATCGCAATC