Insertion junction: LMJ.RY0402.164444_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.164444
Chromosome:chromosome 7
Location:2103928
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g326150 (1 of 34) IPR000571 - Zinc finger, CCCH-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGAGGCGGAAGAGCAACGTCTGATGTTT
Internal bar code:TAGGTCGGACCTGCTCGCGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:953
LEAP-Seq percent confirming:99.8637
LEAP-Seq n confirming:1465
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CGAATTCAGTTTGTCAGCGA
Suggested primer 2:CTCAGCGTGTGTGACCAAGT