| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.164446 |
| Chromosome: | chromosome 9 |
| Location: | 7275194 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412676 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCATCAGTTGCACCCTACGAGACTTTACC |
| Internal bar code: | GCCCGATCTGTCTCTCGTTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1063 |
| LEAP-Seq percent confirming: | 99.5999 |
| LEAP-Seq n confirming: | 3485 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCAGCTCAAACATAGCCA |
| Suggested primer 2: | GCATAACTCGCGTGAGAACA |