| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.164451 |
| Chromosome: | chromosome 16 |
| Location: | 2245711 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658900 | TRP10 | Transient receptor potential ion channel protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCTGCTGGCCACCCACCACGACCCGTTT |
| Internal bar code: | GCAGAGCCCGGCCGTCGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 835 |
| LEAP-Seq percent confirming: | 94.4568 |
| LEAP-Seq n confirming: | 426 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGCCGCATATACTACTGT |
| Suggested primer 2: | GGGAATAGGCAAAAGCAACA |