Insertion junction: LMJ.RY0402.164465_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.164465
Chromosome:chromosome 14
Location:3998269
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g633300 (1 of 1) IPR003582//IPR006626//IPR009030 - ShKT domain // Parallel beta-helix repeat // Insulin-like growth factor binding protein, N-terminal intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTTTACTCGGGTTCTTCACATCAATGGG
Internal bar code:CTAACTACGGACGAAACTCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:96.8708
LEAP-Seq n confirming:712
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCACACACACACACACAC
Suggested primer 2:CGCCAATACACACACACACA