| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.164494 |
| Chromosome: | chromosome 15 |
| Location: | 460337 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636550 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGGTTGAAACAAGTGGCTTTGGAAAGA |
| Internal bar code: | ATTCCCCAAAAGCGGTAATTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 665 |
| LEAP-Seq percent confirming: | 97.0656 |
| LEAP-Seq n confirming: | 2547 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGAAGAACCAAACTTGC |
| Suggested primer 2: | CCGCGGCATACAGTTTATTT |