Insertion junction: LMJ.RY0402.164496_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.164496
Chromosome:chromosome 16
Location:6319904
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g674450 (1 of 4) PTHR11266//PTHR11266:SF42 - PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17 // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCCTCTGCGCCAGTGACGTGGTGTAGGC
Internal bar code:ACCGAGTACGGAAAGGTGGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:661
LEAP-Seq percent confirming:89.1013
LEAP-Seq n confirming:932
LEAP-Seq n nonconfirming:114
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCAGGAGCATGAACCAGT
Suggested primer 2:CATCAAACTTGTGACCCACG