| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.164497 |
| Chromosome: | chromosome 2 |
| Location: | 3743987 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095250 | (1 of 1) 6.6.1.2 - Cobaltochelatase / Hydrogenobyrinic acid a,c-diamide cobaltochelatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCATGGCACTGCAGGTGCGAGCCAGGTCA |
| Internal bar code: | TGTTGCGCAACGATGTTTGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 849 |
| LEAP-Seq percent confirming: | 98.7097 |
| LEAP-Seq n confirming: | 612 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCCAGGTATGAGTGGCT |
| Suggested primer 2: | CTTCTCAGGCTGTTCGTTCC |