| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.164510 |
| Chromosome: | chromosome 11 |
| Location: | 1798327 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467500 | (1 of 1) PF05664 - Plant family of unknown function (DUF810) (DUF810) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTGCGCTCGCTCTCATCGCCCGCAGGA |
| Internal bar code: | AGGCAGCGGCGGGTAGGGTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 746 |
| LEAP-Seq percent confirming: | 99.8493 |
| LEAP-Seq n confirming: | 7951 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGTGGATGTTGGTGAAG |
| Suggested primer 2: | TCACGCAACCTCTCTCACAC |