| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.164511 |
| Chromosome: | chromosome 5 |
| Location: | 3463117 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241450 | FTSY1,FTSY | (1 of 1) K03110 - fused signal recognition particle receptor (ftsY); Chloroplast SRP Receptor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATACCGTGCCCACAGCCCCCTCCCCTGT |
| Internal bar code: | CATCGTAAGCCGCCGGTCAGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1243 |
| LEAP-Seq percent confirming: | 95.1219 |
| LEAP-Seq n confirming: | 78 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGTAGCTCGGAAGGAGG |
| Suggested primer 2: | TTAACGGCGTGCACAGATAG |